Synthesis and Characterization of Nanoparticles Propolis Using Beeswax

In order to protection, convenient release, and increase of antibacterial of capsules to the treatment of diseases, propolis nanoparticles encapsulate. Beeswax is used for covering because of its special physical and chemical properties, ineffective and inactivity and ease of mixingwith materials without any adverse reaction. In this study, nanotechnology and renewable natural compounds of beeswax were used in the process of encapsulating for protection against adverse environmental conditions. At first, propolis nanoparticles were mixed withchloroform then ammonia buffer and Tween -80 was added to it while stirring with speed rpm 300. The mixture was shocked to form the capsule. After filtration andwashing produced capsules were dried for 48 hours at room temperature. Assessment of formation and performance of the capsules was done by changing parameters such as pH, time and temperature, the loading of nanoparticles by spectrophotometry method and increasing the antimicrobial properties using microbial culture.  Also, FT-IR analysis was done to prove the physical transplant of wax and propolis. According to TEM images, the size of produced capsules was estimated in the range of 200 to 500 nm with 95% distribution percentages. Based on Taguchi testing, the optimum time, temperature and pH for the release of encapsulated nanoparticles were 10 minutes, 43ºC and 10, respectively.